IMPACT OF FBXW7 REGULATION ON C-MYC IN MODULATING BIOLOGICAL BEHAVIOR OF ENDOMETRIAL CANCER CELLS: IMPLICATIONS FOR ATHLETE HEALTH MANAGEMENT

Abstract

Objective: To investigate the role of FBXW7 in the malignant transformation of endometrial cancer cells with a focus on its implications for female athlete health. Methods: Expression levels of FBXW7 and C-Myc were measured in endometrial cancer and normal cell lines via Western blot and RT-qPCR. Malignant biological behavior was assessed through in vitro and in vivo assays, examining changes in EMT-related markers like MMP2, MMP9, E-cadherin, and N-cadherin. Results: FBXW7 was significantly downregulated in cancer cells, contrary to the upregulated expression of C-Myc. Enhanced expression of FBXW7 reduced tumor cell proliferation, cell cycle progression, migration, invasion, and increased apoptosis. In vivo, FBXW7 augmentation suppressed tumor growth. Mechanistically, FBXW7 influenced cancer progression by downregulating C-Myc and affected EMT markers by decreasing N-cadherin, MMP2, and MMP9 while increasing E-cadherin expression. Conclusion: FBXW7 serves as a potential regulator of endometrial cancer aggression, impacting proliferation, migration, and invasiveness through modulation of C-Myc. Its study contributes to understanding how molecular mechanisms in cancer could affect athlete health, emphasizing the importance of targeted therapies in maintaining the wellbeing of female athletes.