IMPACT OF HIGH GLUCOSE ON COLORECTAL CANCER CELL PROLIFERATION THROUGH UPREGULATION OF HGF/C-MET GENE EXPRESSION: INSIGHTS FROM ATHLETIC PATIENTS AND MENTAL HEALTH PERSPECTIVES

Abstract

Objective: This study aims to explore the impact of high glucose levels on the proliferation of colorectal cancer cells, focusing on the upregulation of HGF/c-MET gene expression and its implications for athletic patients, considering their dietary habits, physical conditioning, and mental health. Methods: The colorectal cancer cell line SW480 was cultured under varying glucose concentrations. The proliferation activity was assessed using the CCK-8 method, while cell invasion and migration were examined via the Transwell method. Cell cycle distribution and apoptosis rates were analyzed through flow cytometry. Additionally, RT-PCR was employed to measure the mRNA expression levels of HGF and c-MET in the cells. Special attention was given to understanding how athletic patients' unique physiological conditions might interact with these mechanisms. Results: The study found that different glucose concentrations enhanced cell proliferation within the first 24 hours, indicating a slowdown in proliferation rate thereafter. A significant linear correlation between the proliferation of HGF cells and the expression of c-MET was observed post-high glucose induction. Notably, significant differences in cell migration, invasion, and the proportion of cells in the G0/G1 and S phases were identified, alongside a marked variance in apoptosis rates among the groups. The expression levels of HGF mRNA spiked early under high glucose conditions, peaking at 12 hours before diminishing, whereas c-MET mRNA expression reached its zenith at 24 hours’ post-stimulation. These findings suggest a complex interaction between high glucose environments and gene expression dynamics, potentially relevant to athletic patients who may exhibit unique metabolic profiles affecting their cancer risk and mental health. Conclusion: High glucose environments contribute to the proliferation, invasion, and migration of colorectal cancer cells, possibly through the upregulation of HGF/c-MET gene expression. This relationship highlights the importance of monitoring glucose levels and metabolic health in athletic patients, not only for their physical well-being but also for potential implications on mental health and cancer risk management