Enrichment Analysis of Molecular Function and Biological Information of miR-130b-3p Target Gene in Diabetic Nephropathy Among Athletes

Abstract

Objective: To investigate the target gene of miR-130b-3p in athletes with diabetic nephropathy (DN) and analyze its molecular functions, biological processes, and signaling pathways through enrichment analysis.

Methods: Using NCBI, miRbase, and other genomic databases, along with Vector NTI software, target genes of miR-130b-3p were identified and predicted. Enrichment analyses of biological functions and signaling pathways were performed to elucidate the roles of these genes in athletes.

Results: The miR-130b-3p nucleic acid sequence, located on chromosome 22 in Homo sapiens, targets genes involved in critical molecular functions such as tumor necrosis factor activation, cardiolipin binding, and death receptor activity. These genes are implicated in biological processes crucial for athletic performance, including cellular respiration and energy generation. Significant enrichments were observed in cell components like the SAM and MIB complexes, and mitochondrial intima (P<0.01). The KEGG database revealed major enrichment in signaling pathways relevant to athletes, such as TGF-β, lysine degradation, circadian rhythm, and thermogenesis, which have notable biological effects in the context of DN.

Conclusions: The target genes of miR-130b-3p, identified through bioinformatics tools, are predominantly involved in metabolic pathways like TGF-β, lysine degradation, circadian rhythm, and thermogenesis. These pathways play significant roles in the onset and progression of DN in athletes, suggesting that miR-130b-3p and TGF-β pathways could serve as potential therapeutic targets for managing diabetic nephropathy in this population.