INFLUENCE OF ADHESION-REGULATING MOLECULE 1 ON OSTEOGENIC DIFFERENTIATION IN ATHLETIC RECOVERY: AN EX VIVO STUDY IN RAT BONE MARROW MESENCHYMAL STEM CELLS

Abstract

Objective: Investigate the role of Adhesion-Regulating Molecule 1 (ADRM1/RPN13) within the ubiquitin-proteasome system (UPS) in regulating the osteogenic differentiation and proliferation of bone marrow mesenchymal stem cells (BMSCs), highlighting potential implications for bone regeneration in athletic recovery. Methods: Using rat BMSCs, ADRM1/RPN13 expression was manipulated through small interfering RNA for knockdown and expression plasmids for overexpression. The impact on osteogenic differentiation was assessed by quantifying expression levels of key osteogenic markers including collagen I, alkaline phosphatase, osteocalcin, and Runt-related transcription factor 2 (RUNX2) using quantitative reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. Additionally, total ubiquitinated proteins were analyzed, and cell proliferation rates were measured via methyl-thiazolyl-tetrazolium (MTT) assays. Results: Alterations in ADRM1/RPN13 expression significantly influenced the expression of osteogenic markers and proliferation rates in BMSCs. Enhanced ADRM1/RPN13 levels correlated with increased osteogenic activity and elevated expression of RUNX2, suggesting a regulatory role through the UPS. The findings indicate that ADRM1/RPN13 modulates osteogenic differentiation possibly by affecting RUNX2 signaling pathways, highlighting its potential as a therapeutic target for enhancing bone regeneration, particularly relevant in sports medicine for athlete injury recovery. Conclusion: ADRM1/RPN13 plays a crucial role in the osteogenic pathways of BMSCs, with significant implications for bone health and regeneration in athletes. These results support further exploration of ADRM1/RPN13 as a target for therapeutic strategies aimed at accelerating bone healing and recovery in sports-related injuries, potentially offering a novel approach to treat and manage athlete bone health more effectively.