EVALUATING THE IMPACT OF YANGJIN JIEDU DECOCTION ON DIABETIC PERIPHERAL NEUROPATHY AND ATHLETIC PERFORMANCE

Abstract

Background: Diabetic Peripheral Neuropathy (DPN) significantly compromises quality of life and athletic performance due to its debilitating symptoms. Traditional Chinese Medicine, specifically Yangjin Jiedu Decoction (YJJDT), has been posited as a beneficial treatment for DPN, warranting a thorough investigation of its therapeutic effects and mechanisms. Objective: This study aims to elucidate the mechanisms of action of YJJDT on DPN using network pharmacology and to validate its effects in a rat model of DPN, with a focus on its implications for athletes’ health and performance. Methods: The potential targets of YJJDT were identified through network pharmacology analysis, and their interactions with DPN-related genes were examined. The effects of YJJDT on oxidative stress markers and apoptosis in a rat model were assessed by measuring malondialdehyde (MDA), reactive oxygen species (ROS) levels, superoxide dismutase (SOD) activity, and the expression of apoptosis and inflammatory markers through TUNEL assay, flow cytometry, Western blot, and immunofluorescence. Results: YJJDT interacted with 259 predicted targets, including 13 that intersect with DPN-related genes. Key pathways such as the IL-17 signaling and TGF-beta signaling were identified. YJJDT treatment significantly reduced MDA and ROS levels and increased SOD activity, indicating a reduction in oxidative stress. Furthermore, the overexpression of RPS6KB1, a target of YJJDT, was associated with increased neuronal apoptosis and inflammatory response. Conclusion: Yangjin Jiedu Decoction demonstrates promising therapeutic potential in managing DPN by modulating oxidative stress and apoptotic pathways. For athletes, these findings suggest that YJJDT could aid in mitigating the oxidative stress associated with DPN, thereby enhancing recovery and maintaining performance. Future studies should focus on clinical trials to validate these effects in athletic populations, exploring how traditional treatments can be integrated into sports medicine practices.