Background: Lactate Threshold (LT) is characterized by the onset of a rapid increase in blood lactate levels as exercise intensity surpasses the body's clearance capacity. Traditional training for distance runners primarily emphasizes aerobic conditioning designed to enhance cardiovascular efficiency. Purpose: The study aims to evaluate the direct effects of incorporating resistance training on LT, heart rate, pace, and 5K race outcomes in competitive runners. Furthermore, the study explores gender-specific adaptations, filling a gap in the existing literature that has largely overlooked female-specific responses to strength-endurance training models. Methods: This study involved 100 subjects (57 male and 43 female), randomized into experimental (age 24.72±3.26) and control group (age 24.56±3.07). This experimental study was structured as a randomized controlled trial (RCT) with a parallel group design and a 12-month follow-up period. Outcome Measures such as Lactate Threshold, mean Heart Rate, Pace and time taken to Complete 5000m compared at the interval of 0, 6 and 12 months. Results: Experimental group exhibited a sustained and significant increase in LT (mean increase of ~28.2 mg/dl from baseline, p < 0.01). Running pace improved from 3.65 m/s to 3.94 m/s for experimental group. Female participants in the experimental group showed proportionally higher gains in LT (mean +29.7 mg/dl) and 5000m time improvement (mean −92 seconds), suggesting that resistance training confer relatively greater benefits to female endurance athletes. Conclusion: Raising LT helps athletes endure higher intensity efforts over longer periods before fatigue occurs.