Pyroptosis, a form of programmed cell death characterized by inflammation and the recruitment of inflammatory cells, plays a pivotal role in mitigating immunosuppression and sparking systemic immune responses within the tumor microenvironment. The potential of cancer cell pyroptosis to be triggered during radiotherapy, however, remains underexplored. This study delves into the induction of pyroptosis in cervical cancer HeLa cells following irradiation, seeking to pinpoint optimal radiation conditions to incite this process, alongside evaluating the subsequent anti-tumor immune effects. Our findings indicate that exposing HeLa cells to an 8 Gy radiation dose for 72 hours optimally initiates pyroptosis via the caspase-3/GSDME signaling pathway, enhancing phagocytosis and bolstering anti-tumor immunity. This research not only sheds light on a novel approach to cancer treatment through the manipulation of pyroptosis but also opens avenues for investigating the broader implications of this therapy on the mental and physical health of athletes. Enhanced understanding of these dynamics could revolutionize therapeutic strategies, ensuring they support the holistic well-being of individuals undergoing cancer treatment, particularly those in physically demanding professions or activities.