Objective: This paper discusses the effect of vibration training on the strength of knee flexion and extension muscle group of football players. Methods: This test was carried out in a local sports college from January 2021 to January 2022. Taking 22 football players in the college as an example, the athletes were divided into experimental group and observation group by digital grouping method, with 11 people in each group. The observation group was trained with the conventional lower limb physical training method, but also on the equipment. In the process of the experiment, the researchers will focus on the analysis of vibration training on the knee flexion and extension muscle group of football players, and compare the data and effects of the two groups of athletes. The experiment lasted for eight weeks. Results: The data of the experimental group were better than those of the observation group. Vibration training had a significant effect on improving the muscle strength of knee flexion and extension muscle group of football players, P > 0.05. Conclusion: In this eight-week experiment, the vibration training program with 30Hz frequency and 2mm amplitude can significantly improve the muscle strength of knee flexion and extension muscles of college athletes. Compared with the traditional training method, the vibration training program has more advantages, mainly reflected in the tolerance of athletes. However, there is little difference between the method and the traditional training method in the athlete's explosive power, and no great advantage is seen.