The purpose of the present study was to analyze the chronotropic and cardiac autonomic response of a motorcyclist during the 7^th race week of the FIM-CEV Repsol Moto2 European Championship through heart rate (HR_AVG) and sympathetic-parasympathetic ratio (RS-Ps). The effect and relationships of sessions, laps and track segments were analyzed by MANOVA test with Bonferroni post-hoc and Pearson’s r. A low effect of the segments in RS-Ps (p<.01; w_p²=0.03), and high effect of the sessions (p<.01; w_p²=0.72), laps (p<.01; w_p²=0.95) and the interaction between sessions and laps (p<.01; w_p²=0.62) in HR_AVG were found. Also, a tendency of increasing HR_AVG was found with sessions (r=.315; p<0.01) and laps (r=.280; p<0.01). No significant correlations were found with RS-Ps. While HR_AVG can be used as a fatigue index during motorcycling competition, RS-Ps indicates the significant rider’s stress at specific segments of the track, being important both variables for improving the riders’ performance.