The present study aims to assess whether Deep Dry Needling in latent Myofascial Trigger Points is associated with changes in jumping performance. Six Under-21 international beach volleyball players received intervention in vastus lateralis and medialis of the quadriceps. Three squat jump (SJ) and countermovement jump (CMJ) heights were measured in four sessions: pre[1]and post-intervention (acute effect), 48 h and 7 days after intervention (long[1]term effects). The players showed a small decrease after the intervention followed by a non-significant increase at 48 h. However, both jump types resulted in very likely moderate increase after one week of intervention: 4.13 cm (+10.8%) and 3.54 cm (+8.9%) for SJ and CMJ, respectively (3 times the smallest worthwhile change). Despite this significant increase, the decreased performance in post-test results discourages practitioners from using this technique just before a competition.