In this study, the differences in muscle power and blood lactate concentration ([LA- ]b) kinetics among ACTN3 genotypes were examined during the Wingate anaerobic test (WAnT). Thirty-five healthy and physically active men (18-35 years old) volunteered to participate in the study. Muscle power, [LA- ]b concentration and Lactate Dehydrogenase (LDH) activity were analyzed. ACTN3 gene was determined from white blood cell DNA in peripheral blood. Regardless of the participants' body weight or muscle mass, RR genotype carriers developed greater muscle power during Want (p<0.05) than RX or XX genotypes, suggesting that RR genotype has greater capacity to use the ATP-PC system during supramaximal exercise. Muscle power and [LA-]b kinetics were similar in all genotypes. The Want was not sufficient to make muscle damage be detected in LDH concentration.