Objective: To investigate the effect and mechanism of intraneural injection of diosmetin combined with lidocaine on sciatic nerve block in retired players. Methods: Thirty two retired players were randomly divided into four groups: Sham group, diosmetin group (100mg·kg^-1) and lidocaine group (10% lidocaine (20μL)) and lidocaine+diosmetin group (10% lidocaine (20μL) and 100mg·kg^-1 diosmetin). Then the conduction state of sciatic nerve was detected; The sensory and motor function were assessed; the histopathological changes of sciatic nerve were observed by toluidine blue staining; the gene expression of IL-1βand TNF- α on the mRNA level was detected by RT-PCR. Results: Compared with lidocaine group, lidocaine+diosmetin can better enhance sensory and motor nerve block, reduce histopathological damage of sciatic nerve, and decrease the expression of inflammatory factors, such as IL-1 β And TNF- α in transcriptional level. Conclusion: Diosmetin has the effects of enhancing sensory and motor nerve block, anti-inflammatory and protecting nerve injury.