Objective: To analyze the correlation of job efficacy, mental health and job burnout of nursing staff in interventional operating room. Methods: A total of 110 nursing staff in the interventional operating room from August 2020 to February 2023 were selected as the observation objects, and all of them were evaluated by Symptom Checklist-90 (SCL-90), Job Efficacy Scale, and Marquis Burnout Inventory (MBI) to assess their mental health. According to the MBI scale, they were divided into a mild group (n=33), a moderate group (n=59), and a severe group (n=18) to compare the high and low scores of SCL-90 scale and job efficacy scale. Pearson correlation was used to analyze the correlation among job efficacy scale of nursing staff, SCL-90 scale and MBI scale. Results: A total of 100 nursing staff of interventional operating room were all performed by SCL-90 scale with a total score of (222.68 ± 28.61), job efficacy scale with a total score of (23.37 ± 2.58), and MBI scale with a total score of (7.90 ± 1.04). The proportion of mild, moderate and severe job burnout accounted for 30.00%, 53.64% and 16.36% respectively. The low scores of job efficiency of nursing staff in the severe group of job burnout accounted for 83.33%, which was significantly higher than the mild and moderate groups, accounting for 39.39% and 42.37% respectively; the high scores of job efficiency of nursing staff in the mild group of job burnout accounted for 69.70%, which was significantly higher than the moderate group and severe group, accounting for 38.98% and 38.89%, respectively and the difference was statistically significant (P<0.05). Pearson correlation analysis showed that job efficacy scale was significantly negatively correlated with MBI scale of nursing staff (r=-0.513, P<0.05), and SCL-90 scale was significantly positively correlated with MBI scale (r=0.627, P<0.05). Conclusion: Job burnout of nursing staff in intervention operating room is closely related to job efficacy and mental health. It is suggested to focus on the psychological status and the sense of job efficacy of nursing staff in intervention operating room to create a good professional environment and improve the quality of nursing personnel training.