Objective: To evaluate the effectiveness of combining electrophysiological assessment and sports-based rehabilitation therapy in treating pelvic floor dysfunction (PFD) among athletes. Methods: This study included 102 athletes diagnosed with PFD who were treated at our sports medicine facility from January 2022 to January 2023. Participants were randomly assigned (envelope method) to either a control group receiving standard rehabilitation therapy or a study group undergoing a combination of rehabilitation therapy and electrophysiological techniques. We assessed and compared outcomes including muscle strength, urinary incontinence, organ prolapse, and various pelvic function indices between the two groups. Results: The study group, which combined electrophysiological techniques with rehabilitation therapy, showed significantly improved muscle strength (P < 0.05), better control of urinary incontinence (P < 0.05), reduced organ prolapse (P < 0.05), and enhanced overall pelvic functional indices post-treatment (P < 0.05), compared to the control group. Conclusion: Integrating electrophysiological assessments with targeted sports-based rehabilitation therapy significantly enhances treatment outcomes in athletes with pelvic floor dysfunction. This approach not only improves specific pelvic metrics but may also contribute to overall athletic performance and quality of life. Future research should focus on refining these combined therapies and exploring their long-term benefits on athletic health and performance.