Objective: This study aims to explore the role of long non-coding RNA (lncRNA) SNHG9 in enhancing rehabilitation outcomes for sports-related endothelial damage, with a focus on the AMPK/SIRT1-PGC-1α pathway in the context of physical therapy. Specifically, it investigates the expression of SNHG9 in human umbilical vein endothelial cells (HUVECs) under oxidative stress conditions, which are analogous to endothelial damage observed in athletes with musculoskeletal injuries. Method: HUVECs were cultured and observed under an inverted microscope, divided into groups based on varying concentrations of ox-LDL treatment, a model for oxidative stress. The study utilized Western blot analysis to measure the expression levels of SNHG9 in response to ox-LDL, and the effects of SNHG9 on HUVECs under oxidative stress were observed. Key markers such as glutathione peroxidase (GSH-PX), superoxide dismutase (SOD), lactate dehydrogenase (LDH), Malondialdehyde (MDA), and proteins Bcl2 and Bax were measured to assess cell damage and protective mechanisms. Results: Normal HUVECs exhibited typical morphology, and exposure to ox-LDL significantly increased the expression of SNHG9, especially at the highest concentration and longest exposure time. SNHG9 overexpression led to increased activities of SOD and GSH-PX, upregulation of Bcl2, and reduced levels of LDH and MDA, indicating protective effects against oxidative stress. Conversely, inhibition of SNHG9 expression resulted in reduced protective enzyme activities and increased cell damage markers. The role of the AMPKSIRT1 inhibitor in this process was also elucidated, showing its significant impact on SNHG9 expression and related protective mechanisms. Conclusion: The study concludes that lncRNA SNHG9 plays a critical role in mitigating endothelial cell injury caused by oxidative stress, relevant to sports-related injuries in athletes. This protective role of SNHG9 is closely associated with the AMPK/SIRT1-PGC-1α signaling pathway, suggesting its potential utility in enhancing rehabilitation outcomes in physical therapy for athletes suffering from sports-related endothelial damage. These findings pave the way for targeted therapeutic strategies, leveraging the modulation of SNHG9 to improve recovery and rehabilitation in athletic patients.